Sunil Pachakar

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Questions related from Sunil Pachakar

Can anyone suggest a protocol to purify cell free DNA fragments less than 200 bp using AmpureXP beads?

I regularly purify cell free DNA from patient plasma samples using Qiamp circulating nucleic acid kit, followed by analysis on BioAnalyzer which indicates genomic DNA contamination in few of the...

05 January 2019 1,939 2 View

How to calculate copies of circulating tumour DNA from sequencing data?

NGS data provides information on variant allele frequency which is to be used for calculating copies of total cf-DNA, variant copies of cf-DNA per ml. I have used the method described in Annals...

29 November 2017 8,151 2 View