Can anyone suggest a protocol of protein deglycosylation using the TFMSA (trifluoromethanesulfonic acid) please?

More Marta Mikolajczak's questions See All

Does anyone know a robust protocol for performing 'native' Western Blots?

By 'native' I mean no beta-mercaptoethanol and no SDS. Basically I would rather not disrupt any bonds that can be forming in terms of my protien of interest. I would rather make it in the lab than...

09 October 2014 1,071 8 View

Does anyone perform immunolabelling of live cell cultures?

Hello, I am trying to perform immunolabelling of live cell cultures, I am looking at a secreted protein, that hopefully gets trapped at the cell surface. At the moment I am struggling with...

07 August 2014 449 11 View

Monolayer of primary neural stem cells?

So far I have used the neurosphere assay for my experiments but due to many drawbacks of this assay I would rather use a monolayer cell culture of neural stem cells. Has anyone successfully grown...

09 October 2013 6,270 11 View

Can someone advise on the use of tamoxifen in vitro in a cell culture?

I would like to set up an in vitro culture of cells that originate from a CreERT2 transgenic mouse. Normally this system is activated in vivo with tamoxifen but I would like to induce this system...

09 October 2013 2,681 9 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Bassel Sabbagh

you can try this one, it is a protocol for isolation and enrichment of secreted protein from filamentous fungi, including chemical (using TFMSA) and enzymatic deglycosylation.

http://www.ncbi.nlm.nih.gov/pubmed/18369903

Marta Mikolajczak

I think this might work, I will give it a go, thank you!!!