Marta Mikolajczak

5 Questions 19 Answers 0 Followers

Questions related from Marta Mikolajczak

Does anyone know a robust protocol for performing 'native' Western Blots?

By 'native' I mean no beta-mercaptoethanol and no SDS. Basically I would rather not disrupt any bonds that can be forming in terms of my protien of interest. I would rather make it in the lab than...

10 October 2014 1,134 8 View

Can anyone suggest a protocol of protein deglycosylation using the TFMSA (trifluoromethanesulfonic acid) please?

TFMSA protein deglycosylation.

08 August 2014 5,052 2 View

Does anyone perform immunolabelling of live cell cultures?

Hello, I am trying to perform immunolabelling of live cell cultures, I am looking at a secreted protein, that hopefully gets trapped at the cell surface. At the moment I am struggling with...

08 August 2014 510 11 View

Can someone advise on the use of tamoxifen in vitro in a cell culture?

I would like to set up an in vitro culture of cells that originate from a CreERT2 transgenic mouse. Normally this system is activated in vivo with tamoxifen but I would like to induce this system...

10 October 2013 2,755 9 View

Monolayer of primary neural stem cells?

So far I have used the neurosphere assay for my experiments but due to many drawbacks of this assay I would rather use a monolayer cell culture of neural stem cells. Has anyone successfully grown...

10 October 2013 6,332 11 View