I m afraid that RNA is long gone from your samples. However the durability of DNA is far greater. I suggest to try a simple DNA purification method (using a kit) and see how it goes.

Thanks for your suggestion. This is what I anticipated too about the RNA. But I was looking for an inexpensive way to extract nucleic acid especially DNA. Kits are too much expensive.

Hi Muhammad,

I'm not sure if it will help but in the method we used for extracting from granular sludge (DNA/RNA co-extraction). Sure your RNA will be gone but you could still use this method. We used a liquid nitrogen pestle and mortar to grind the sludge (you could use this to grind the mushrooms), then added to lysing matrix tubes with extraction buffer (you will have to test different quantities to see what works best) and vortex for ten minutes then proceeding to use an altered version of the Phenol-Chloroform extraction method.

Hope this helps,

Ciara

Thanks Ciara, I will try this one too and will let you know if it works. Cheers