Can anyone suggest a method to quantify different kinds of fatty acids other than CG?

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Electroporation parameters for jurkat cells using neon system?

Hello! I have tried to electroporate the Jurkat cell line with the Neon System using the parameters provided by the supplier, but almost all the cells die after the procedure. I have also tried...

10 April 2024 1,382 0 View

¿Cómo puedo informar correctamente aceerca de un posible caso de trata?

Todo el mundo alega que si ocurriese una injusticia lo correcto sería contarlo pero, cómo se debería dar voz de forma adecuada en este caso, sin poner en riesgo a las víctimas?

10 March 2024 5,202 1 View

¿Es lo mismo trata de personas que tráfico de personas?

Normalmente se suelen utilizar esos dos términos coloquialmente a la hora de hablar sobre el tema, y me gustaría saber las diferencias para llevar el tema con propiedad y que más personas sean...

10 March 2024 2,567 1 View

Is it possible to use the Emotiv Flex (either the Saline or Gel version) for Neurofeedback sessions?

Additionally, what software can be used for training that is compatible and easy to set up with this device? Thanks

16 January 2024 3,464 0 View

Why doesn't fsolve in Maple return all complex roots of a polynomial?

Why does fsolve(x - (-8.0)^(1/3), x) returns only the first root (1.000000000 + 1.732050807*I) of the polynomial (which clearly has 3 roots)?

28 December 2023 8,244 0 View

Does anyone, by any chance, have the datasheet from the HPLC column Pharmacia Sephasil Peptide C18 5u ST 4.6x250 mm?

Hello everyone! I recently decided to run some tests on an old HPLC C18 column but I'm unable to find its datasheet. Even though I know wich buffers to use, I really would appreciate working...

30 August 2023 8,368 2 View

Can anyone explain the morphology found in astrocyte labeling with GFAP?

Using the same antibody to mark astrocytes, we found markings with commonly known shapes, but we also found markings similar to the image below, both in the same animal and in other animals of...

17 August 2023 4,091 2 View

How to develop the Mander curve of a concrete column?

I need to perform the manual calculation but I am confused about the formulas and steps to follow for the elaboration of the Mander Model in a concrete column

18 June 2023 5,340 1 View

Why did the cells detached in a sheet-like form?

Hi, I encountered an issue while detaching some cells. I had both HEK 293 and RAW 264.7 cells. When I added 0.05% trypsin, the cells did not round up and instead detached in the form of a sheet....

01 June 2023 1,304 3 View

How to decrease the amount of white blood cells obtained from a murine hepatocyte extraction?

As a final project for a college course, we need to do a primary culture of non parenquimatic liver cells from mice. In our first try we obtained a great amount of white blood cells instead of...

14 May 2023 9,576 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View