We obtained essential oils as a result of direct distillation of raw water vapor. By FE most raw materials should distill with the addition of xylene. Which reagents should be used to examine the activity of such oils by becoming xylene? How can one properly prepare such essential oils to determine the common methods for comparing the activity of DPPH, FRAP, ABTS, ORAC?
May be defined as volatiles, odoriferous oils of vegetable origin. Oil extracted
by solvent extraction retain the component of the oil better than the one extracted by distillation. The compounds occurring in essential oils are mostly
(i) Esters of benzoic, acetic and cinnamic acids.
(ii) Alcohols: Geraniol, citronellol, menthol.
(iii) Aldehyde: Citral, citronella, vanillin, benzaldehyde.
(iv) Acids e.g. benzoic, cinnamic.
(v) Phenols: Eugenols, thymol.
(vi) Ketones: Carvone, methone, camphor.
(vii) Ethers: Anethole, Safrole.
(viii) Lactones: Coumarin
(ix) Terpnes: Camphene, pinene
(x) Hydrocarbon: Styrene. Volatile oils may be recovered by (1) Distillation (2) Extraction with volatile solvents. During extraction process xylene get evaporated distillation of the oil, because it has low boiling point. There are chances of partial mixing of components if surface slurry is used or added to get absorption of oils at low temperature. You can go for further distillation essential oils to obtain them pure to determine the activity of DPPH, FRAP, ABTS, ORAC
Dear Ravi,
thanks for the suggestion, so obviously the volatiles may be obtained by extraction using different solvents. However, using the method of distillation, because of this I mean, I have some problems with xylene, which as you know is used in pharmacopoeial methods, which in turn must be maintained during testing of herbal and pharmaceutical raw materials.
You mean "overcoming" (= removing) xylene, right ? and NOT "becoming" xylene? If so, just remove xylene again in a rotary evaporator and then can reconstitute the essential oil components in 5% or higher %age DMSO or Methanol or Ethanol and then perform the antioxidant activities. I have determined similar tests which are provided in our published manuscript as well. please let me know if you want further clarifications as well. best wishes.
Dear Biswapriya Biswavas Misra
Dziękuję za propozycję rozwiązania problemu i pomocny artykuł. Oczywiście mówię o usunięcie ksylenu z mieszaniny olejku. Kiedy stosować proponowane rozwiązanie chcieliby skorzystać nawet z pomocy w przypadku jakichkolwiek pytań.
Z poważaniem
Medicinal plants are known to possess many different components which have various biological activities. The dried leaves of Globularia alypum were treated by hydrodistillation and the aqueous phase was exposed to fractionation by polar or non polar solvents. The chemical analysis of petroleum ether extract (11.73%, w/w) isolated from Globularia alypum leaves by CPG/ms showed 82.36% and 17.64% as major and minor total identified compounds, respectively, presenting high fluctuations in its chemical profile. The results revealed relatively a high content of xylene (11.72%) among 31 identified compounds
http://jocpr.com/vol6-iss12-2014/JCPR-2014-6-12-776-784.pdf
Dear Krishnan Umachandran thanks for the helpful solution.
I've always wondered whether it is possible to eliminate m-xylene or not it affects the image of DPPH oils and extracts. Because of course, some oils may be distilled only with the addition of m-xylene.
Global Meta-xylene market to grow at a CAGR of 4.64% over the period 2014-2019.
Availability of efficient and advanced recovery processes to obtain high-purity meta-xylene is a positive trend in the market. Advanced recovery processes such as simulated moving bed adsorption and crystallization help the recovering of meta-xylene from mixed xylenes feedstock. These processes offer several distinct advantages such as obtaining high-purity meta-xylene up to 99.5 percent, requirement for less equipment, use of carbon steel metallurgy, extended adsorbent life up to 10 years, and high on-stream factor for reliable operation.
http://www.prnewswire.com/news-releases/global-aroma-chemicals-market-2019---natural-and-synthetic-aroma-chemicals-analyzed-295622741.html
Check if this publication is useful
https://ttngmai.files.wordpress.com/2012/09/handbookofessentionaloil.pdf
Honestly, I have lost the meaning of the question. If you need to evaluate oil activities, such as antioxidant one, and the presence of solvents as xylene/toluene is not desirable, there are different options to obtain the volatile compounds without use this kind of solvents.
But, if the question is how to measure antioxidant activities in the presence of this kind of solvents, the answer will be different.
Could you resolve your problem? do you need more information?
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