Bender,

If you need to identify starch (qualitative method), you can use Lugol (IKI, iodine-potassium iodide) or the PAS reaction (total polysaccharides). However, both methods are not useful to identify callose. So, under fluorescence microscopy, you must use Aniline Blue.

Thanks Rinaldo - I am looking for quantitative methods.

Renar,

This paper may help you.

Dr Zhang, thanks for the paper and I have to apologize for having postponed so much sending you a return message. Best regards, Rjbender

Dear Renar,

I analysed starch in roots and tubers using complete acid hydrolytic method followed by Lane and Eynone method to analyse the total starch. You can get my relevant papers from research gate. Conveniently you can use Megazyme starch assay kits for quantification of starch. 

Dear Suraji, many thanks for your answer. I will through your work published in RG. Best regards, Rjbender