I m working with PCV2 detection in SERUM of pigs (sus scrofa).
I need to know the vality of false positive or false negative results of my samples, and the integrity of my DNA samples
I m using taqman probe,
There is a lots of old information and a couldn't find the good internal control amplification for my situation.
ex. ACTB, GAPDH, 18S
Mr Prabu, thanks,
I already used a standard plasmid spiked in my samples, and there is inhibition. I'm using absolute quantification (qPCR).
Is there any constitutive gene (like ACTB, GAPDH, 18S) in pigs serum that I can use for confirmation of my results? I need check the integrity of my DNA extracted samples and compare to my target results.
Mr Prabu, thanks,
I already used a standard plasmid spiked in my samples, and there is inhibition. I'm using absolute quantification (qPCR).
Is there any constitutive gene (like ACTB, GAPDH, 18S) in pigs serum that I can use for confirmation of my results? I need check the integrity of my DNA extracted samples and compare to my target results.
GAPDH might be the most fitable one for you. I've used GAPDH as internal control for DNA extracted from pig serum just like you and it worked well. But I used SYBR green at that time. there is 18s rRNA kit from applied biosystem and it is for probe type qPCR. It was tagged woth VIC and working very well.
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