I need to purify recombinant proteins from a culture medium using HisTag. I have some experience in purification of bacterial proteins but this task looks rather different and more complex. I would be grateful for any links for guides or advise.
Thank you, Ted!
I'm writing a Python code which uses R scripts by calling them trough subprocess.run(). The R script I try to call works as supposed when I call it from PowerShell by command: &...
07 August 2019 3,431 3 View
Working with home-made sandwich ELISA I found out a strong dependency between measured antigen concentrations and sample dilution factor. I have attached one of such figures. Several blood plasma...
08 September 2017 6,529 24 View
Is there any approach to reduce inter-assay variability of home-made sandwich ELISA except good pipetting practice? Thank you for any suggestion!
05 June 2017 7,509 6 View
I'm trying to compare cell diameters and volumes of different cell types using an automated cell counter Scepter (Millipore). The counter draws histograms of diameters and volumes on the screen...
05 June 2017 5,517 6 View
10 November 2016 4,449 6 View
I purified recombinant protein (expressed in E. coli) 6 months ago and stored it at room temperature in 8 M urea. Previously I observed a thick band of the protein in acrilamide gel. Now the band...
02 March 2014 9,856 25 View
Recently I generated stable SP2/0 cells carrying my gene of interest. I used pcDNA6/v5 His A as a vector and blasticidin S as a selective antibiotic. The vector was delivered into cells by...
01 February 2014 1,074 3 View
Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1
17 August 2021 0 0 View
20 June 2021 0 0 View
--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?
24 March 2021 0 0 View
I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...
03 March 2021 4,116 4 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I looking for ferro calcium production process
03 March 2021 1,755 4 View
Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,
02 March 2021 0 0 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View