Ilya Smirnov

8 Questions 30 Answers 0 Followers

Questions related from Ilya Smirnov

Why is R script unable to load ggplot2 package when called by Python subprocess?

I'm writing a Python code which uses R scripts by calling them trough subprocess.run(). The R script I try to call works as supposed when I call it from PowerShell by command: &...

08 August 2019 3,535 3 View

Why do antigen concentrations measured in ELISA change depending on sample dilution factor?

Working with home-made sandwich ELISA I found out a strong dependency between measured antigen concentrations and sample dilution factor. I have attached one of such figures. Several blood plasma...

09 September 2017 6,649 24 View

Reduction of inter-assay variability of home-made ELISA

Is there any approach to reduce inter-assay variability of home-made sandwich ELISA except good pipetting practice? Thank you for any suggestion!

06 June 2017 7,595 6 View

Cell size and volume determined by Scepter (Millipore)

I'm trying to compare cell diameters and volumes of different cell types using an automated cell counter Scepter (Millipore). The counter draws histograms of diameters and volumes on the screen...

06 June 2017 5,612 6 View

Is there any special technique for urine sample freezing?

I’m looking for a special technique for freezing of urine samples for analysis by ELISA. If I test blood plasma samples even multiple freezing-thawing cycles do not affect an antigen...

11 November 2016 4,514 6 View

Can anyone suggest a good guide for recombinant protein purification from mammalian cell cultures?

I need to purify recombinant proteins from a culture medium using HisTag. I have some experience in purification of bacterial proteins but this task looks rather different and more complex. I...

04 April 2014 3,177 1 View

What is the reason of protein degradation in 8 M urea?

I purified recombinant protein (expressed in E. coli) 6 months ago and stored it at room temperature in 8 M urea. Previously I observed a thick band of the protein in acrilamide gel. Now the band...

03 March 2014 9,921 25 View

Could anyone help me how to enhance protein expression in stable SP2/0 cells?

Recently I generated stable SP2/0 cells carrying my gene of interest. I used pcDNA6/v5 His A as a vector and blasticidin S as a selective antibiotic. The vector was delivered into cells by...

02 February 2014 1,141 3 View