if you use JAX-OT-I, you can use the protocols described on web page for standard PCR: https://www2.jax.org/protocolsdb/f?p=116:5:0::NO:5:P5_MASTER_PROTOCOL_ID,P5_JRS_CODE:22848,003831

or QPCR: https://www2.jax.org/protocolsdb/f?p=116:5:0::NO:5:P5_MASTER_PROTOCOL_ID,P5_JRS_CODE:22187,003831

Is important that you perform any functional assay for CD-8 cells by FACS.

Thanks Catalina Pardo-Roa

Dear Asthma,

you need first to decide the sampling method, then looking for a suitable protocol for this. For example, a sample from the mouse tail then follow this protocol:

http://www.abnova.com/abvideo/Mouse-Genotyping.html

Hope it helps !

thanks Eman Bassiony