Can anyone suggest a better method to grind wheat seeds for total RNA isolation?

21 October 2019 1 7K Report

I used mortal and pestle to grind the seeds in liquid nitrogen however could not get fine powder and so obtained a lower RNA yield.

Alexander Sasha Vlassov

alternative is bead beating- with high power bead beating instruments available from Omni, OPS Diagnostics, several other companies. The sample is placed in a tube with steel, zirconia or glass beads (material and bead diameter varies, as well as amount/tube) and homogenized by vigorous shaking

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