Prafful Sharma

3 Questions 1 Answers 0 Followers

Questions related from Prafful Sharma

Can a triax biocomp fractionator be used to detect fluroscently labelled protein-RNA complex separated via a sucrose density gradient?

Has anyone done any fluorescent-based detection assay using this machine? Is there a protocol? Which fluorescent dye among Cy3 and fluorescein will be suitable (give a high signal over the...

03 July 2023 2,729 0 View

Why is the potential energy positive and not declining exponentially after the energy minimization step in GROMACS?

I am trying to simulate a protein with a peptide in GROMACS using CHARMM36 force field and TIP3 recommended water model. After the EM step I see that the potential energy is positive and the curve...

25 November 2021 4,709 1 View

Can anyone suggest a better method to grind wheat seeds for total RNA isolation?

I used mortal and pestle to grind the seeds in liquid nitrogen however could not get fine powder and so obtained a lower RNA yield.

21 October 2019 7,128 1 View