Dear Swetha,

I do not have my own pistures, but on this web page you can see one photo: http://library.med.utah.edu/WebPath/CINJHTML/CINJ040.html

Regards, Ania

Seconding Anna Maria, I would like to provide some sources more: :

i) https://upload.wikimedia.org/wikipedia/commons/thumb/c/c0/Ground_glass_hepatocytes_high_mag_cropped.jpg/1024px-Ground_glass_hepatocytes_high_mag_cropped.jpg (poor quality, only vizualized as brown blurrred granular appearance  within cells);

ii) https://en.wikipedia.org/wiki/Lipofuscin#/media/File:Cardiac_myocyte_showing_lipofuscin_pigment.jpg

iii) perhaps most interesting (discrimination of L. by use of other staining technique, use the CTRL-F function for searching "lipofusc"[but wait until the whole pdf has loaded properly] :   p 37 / 158:  image/picture: 

Figure 4. Lipofuscin in cells of the liver.

Gomori’s aldehyde-fuschsine, applied after oxidation with potassium permanganate and treatment with oxalic acid to remove deposited MnO2. The counterstain is tartrazine, a yellow anionic dye. [ http://www.dako.com/08066_guide_to_special_stains.pdf]

and last but not least [not in liver, but in prostata],

iv) e.g. Pigment in prostatic epithelium, slide 22 of 24

(image-legend) Comments:

The presence of pigment is a common finding in prostatic secretory epithelium. The pigment is usually granular and may be yellow-brown, brown, gray, blue-purple, or almost black. The granules may be apical or subnuclear. The amount and the presence of pigment is highly variable within a biopsy core. Based on the biochemical properties and staining characteristics, the pigment appears to be Lipofuscin and consists of oxidized lipids.

cf: http://www.webpathology.com/image.asp?n=22&Case=14  or:

http://www.webpathology.com/image.asp?case=14&n=22  and 

http://www.webpathology.com/image.asp?case=14&n=23, respectively.

Best wishes and good luck, WM

Sir, 

This is in context with the above mentioned techniques to highlight the lipofuscin pigment. Can you please elaborate on how the process of Long ZN stain different from the conventional stain? 

Regards

Prita

And dear Shweta,

Coming to your primary question. On H &E, lipofuscin looks golden yellow to brown in colour. You can look for them in the cytoplasm of the hepatocytes. And they tend to have a powdery or granular appearance.

Regards 

Prita

Thank you all for your valuable insights. I shall keep you posted about my progress in the area and hopefully you all shall help me out in future

 Thanx again.

I recommend you to buy  an atlas of fish histology.For example:

1 Hugh W.Ferguson.A text and atlas of normal tissues in Teleosts and their response in diseases.

2.Kubota S., teruo Miyazaki,Syuzo Egusa.Color Atlas of histopathology

3/David Bruno,P.Noguera,T.Poppe.A color atlas of salmonid diseases.