I am working with a team looking into testing antiviral activity of naturally occurring substances. We have not decided on the specific protocols but I am leaning towards the plaque reduction neutralization assay. I'm hoping you might share your protocols or point me to a reference. Preferably one using Vero cells.
Thanks!
Ferdinand E. Mendoza
A micro-plaque assay methodology for quantifying neuraminidase inhibitor resistance has been developed by Matrosovich et al (2006) which overcomes some of the difficulties of conventional overlay methods such as agar, which cannot be used in 96-well plates. The method utilises Avicel™ (microcrystalline water insoluble cellulose) which produces larger and more distinct plaques. This has the advantage of increasing the sensitivity of the assay and reducing the incubation time. A simple, inexpensive method for quantitfying the results has recently been developed by Sullivan et al (2012)
Virapur provides an assay:
https://www.virapur.com/protocols/Virus%20Plaque%20Assay%20Protocol.pdf
Hi Ferdinand
Hope this article helps you
https://virologyj.biomedcentral.com/articles/10.1186/1743-422X-10-245
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