Can anyone share the proper method for phytochemicals extraction and how long should we keep the sample for solvent drying in hot air oven?

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Ankit Negi

try water bath.

Mubashir Iqbal

The method for phytochemical extraction can vary depending on the type of plant material and the specific phytochemicals of interest. However, here is a general protocol for phytochemical extraction:

Preparation of plant material: Wash and dry the plant material thoroughly. Grind or chop the material into small pieces for increased surface area.

Solvent selection: Choose an appropriate solvent or solvent mixture based on the type of phytochemicals you want to extract. Common solvents include ethanol, methanol, acetone, and water.

Extraction process: Combine the plant material and solvent in a flask or beaker and let the mixture sit for several hours to allow for extraction. Agitate the mixture periodically to enhance extraction efficiency.

Filtration: Filter the mixture to remove any solid plant material and impurities.

Solvent evaporation: Use a rotary evaporator or a hotplate to evaporate the solvent from the filtered solution.

Drying: Dry the residue obtained after solvent evaporation in a hot air oven at a suitable temperature for a suitable time, depending on the solvent used and the nature of the sample.

The time required for solvent drying in a hot air oven can vary depending on the nature and amount of the solvent used. Typically, a temperature between 40-60°C and a drying time between 4-8 hours are appropriate for most plant materials. However, it is important to monitor the sample closely to avoid over-drying or burning, which can degrade the phytochemicals of interest.

Mukesh Ananta Chaudhari

Honey Gupta ji, will you please let us know which drug you are using?Soxhlet Extraction is also a good method for extraction with diluted ethanol.

@ Honey Gupta https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398001/

Hamid Reza Farhang

Some extraction methods, such as percolation, Macerassion and Soxhlet, can be useful. However, according to the type of secondary metabolite, The best method of them should be tested and selected using experiment (trail and error).

Regards