Best analysis for this longitudinal study?

Hi everyone, I need help for what analysis is better for this longitudinal study. In my study, I measured the attachment security of children at 5 time points. My dubts are that: 1) spacing...

07 June 2020 9,834 2 View

Cardiac rupture following electrocution in birds: is it common?

24 November 2019 9,676 2 View

Could you suggest a microplate reader for bacterial kinetics?

I would like to purchase a microplate reader to assay bacterial growth kinetics when exposed to different conditions (i.e.:salt concentration, different sugars, etc.). Ideally, I am looking for an...

11 September 2019 3,129 2 View

Did someone use an antibody against caspase-9 for immunoprecipitation from mouse cell lysates?

14 December 2015 9,546 2 View

Can anyone suggest some good laboratories in Northern Italy for standard environmental testing?

I need to identify some good Labs placed in Northern Italy to be able to make vibrations, thermal cycling, ingress protection, salt spray and cold soak tests for automotive application (and not...

11 November 2015 5,518 5 View

Who are the worldwide experts/opinion leaders in primary cancer cells 3D culture?

25 October 2015 1,063 3 View

Does Someone know if FITC is soluble in chloroform?

I know it is soluble in ethanol, acetone, DMSO, DMF; someone knows about chloroform?

19 March 2015 4,740 2 View

Why don't my EBV transformed B cells secrete antibody?

Dear all, I transformed with EBV B cells from total PBMC and after 1 month I isolated IgG+ cells using Miltenyi beads. Keeping in culture these cells with CpG and without, I got proliferation but...

16 March 2015 9,839 5 View

Does anyone know which primers should I use to genotype CSHL gene trap lines?

I am trying to genotype some CSHL gene trap lines in A.thaliana and I am having some difficulties in designing the primers. For those on the flanking regions (ie, the LP and RP), I used the iSct...

12 February 2015 2,011 3 View

Fluorescein stabilization in ethanol solution for temperature measurements

I'm working since several months with a solution of Fluorescein Disodium (C20H10Na2O5) and Ethanol. So far, the spectrum of this solution is changing due to unknown parameters and I would like to...

01 January 1970 7,629 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View