How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Why Arnold Cat Map is widely used to encrypt the watermark?

What is the main reason that most of the researcher uses ACM to encrypt the watermark? There is various other image encryption technique but for watermarking ACM technique is used?

24 December 2020 9,807 1 View

Which polar protic solvent are used in replacement of Ethanol or methanol?

In reaction condition, my starting materials are highly polar which are not soluble in ethanol and methanol.

03 December 2020 5,792 2 View

Can anyone recommond the journal for biopolymer production?

I wanted to know about the journal with a high rate of acceptance and sci index.

01 December 2020 7,533 1 View

How can I model Solar PV, Battery from Electric vehicles, and grid in Matlab for cost minimization?

How can I model a system in Matlab consisting of Solar PV, Several Batteries from Electrical vehicles, and a grid altogether so that the load demand is fulfilled in such a way that the cost of...

20 November 2020 8,580 4 View

How can I model three types of power sources which are fossil power from the grid, green power from the solar panel, and green power from the PHEV?

How can I model three types of power sources which are fossil power from the grid, green power from the solar panel, and green power from the PHEV from cars of 100 employees (who come in the...

12 October 2020 4,720 3 View

How many internet Switches are there between one country to another country?

How many internet Switches are there between one country to another country?.

11 October 2020 1,034 3 View

Is "Toxic Positivity" is harming Teachers during this Pandemic?

School leaders are trying to infuse positivity in teachers through the toxic positivity. It is creating more frustrations in teachers because their problems are not solved yet expected to work...

11 October 2020 8,210 4 View

What should be the Bragg R-factor value to stop at while performing the Rietveld Refinement?

How does the Bragg-R factor value effects the chi-square value?

22 September 2020 5,784 4 View

Dispersion of Graphene?

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14 September 2020 8,364 5 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View