Can anyone share a best tutorial for scRNA data handling from starting to end using R tutorial?

26 May 2020 1 10K Report

Hi all, I have scRNA data generated from Rapsody platform and analysed in seven bridges platform. Now could you please give me an idea how to deal with seven bridges platform output files for seurat R scRNA analysis. Mainly i need Filtered Feature, Barcodes, and Matrix files for analysis.

Sagar Sanjiv Desai

You would have obtained MolsPerCell.csv and Samples_Tags, among other files, from Seven Bridges.

Just follow the script given at this link

https://github.com/MairFlo/Targeted_transcriptomics/blob/master/Mair%20and%20Erickson%20R%20Script%20Part1%20(Seurat2.3).R

it should do the job. Let me know if you need more clarification

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