What would be standard formula/equation to derive post-test mean & standard deviation values from the established study for meta-analysis?

Since in some studies, authors only report the mean difference and mean difference standard deviation. Kindly, help me out to get the absolute mean and SD of post-test measures. Thanks

22 March 2020 5,358 7 View

Why cell proliferation increased after change amino acid by SDM?

Dear Scientists, I am working with a gene of interest in colorectal cancer, which has no role in cell proliferation. In addition, by knockdown this gene, the FAK and SRC protein expression are...

09 October 2019 6,257 3 View

I did emulsion polymerization using 2-carboxyethyl acrylate as co monomer. When I increased the amount of CEA, particle size increases. Any idea?

I did emulsion polymerization using 2-carboxyethyl acrylate as co monomer. When I increased the amount of CEA, particle size increases. I found out that at higher CEA content, particle size is...

03 July 2019 4,764 4 View

Does anybody know a company specialized in platinum machining?

Good day! Does anybody know a company providing on-demand platinum machining service? Thank you in advance for your help! Kind regards, Reda

14 February 2019 9,275 3 View

Is there any indexed journals with fast publication process that cover the field of computers and environment?

Hi, I am looking for journals with good impact factor that cover the scope of modern technology impact (e.g. computers or computer networks) on saving the environment. regards.

20 September 2018 7,765 3 View

Emojis and text analysis?

I would like to hear from those with interest (with strong technical skills) in Emoji and text analysis in social media feeds for potential collboation on a paper.

06 September 2018 3,113 5 View

How to prepare a conditioned media post transfection with specific plasmid ?

Hi Researcher, I am new PhD student and I have plan to start an experiment which is requires to do different techniques. Therefore, I would like any researcher find my post to answer my question...

20 May 2018 9,526 2 View

Which is the perfect (DNA isolation or RNA isolation) in the expression of gene having intron?

gene expression in plant looking for any change in the sequence after plant exposure to stress conditions ... gene size = 1007 bp

25 October 2017 3,256 6 View

How do you correctly interpret Gel Electrophoresis results?

Please view the uploaded file, for the Obtain image that i acquired from the completion of the practical.

22 October 2017 1,880 3 View

QPCR: analyses involving in-between plates?

hi i am not sure if it is alright to analyse my data this way. So i prepared 3 samples of controls, 3 samples of condition at 1hr exposure, 3 samples of condition at 6hr of exposure. i collected...

14 August 2017 8,631 4 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View