We are repeatedly troubled with strange Protein bands in PAGE after PCR using Q5 polymerase from NEB. Who has measured the molecular weight of Q5 already and could help us out? Thank you all in advance,
"Q5 DNA Polymerase is composed of a novel polymerase that is fused to the processivity-enhancing Sso7d DNA binding domain, improving speed, fidelity and reliability of performance. "
To clarify, we observe additional bands in PROTEIN gels and would like to verify whether one of These bands is Q5. Maybe anyone has already determined the molecular weight of Q5, so we could rule out that the contamination is due to Q5?