I hope this finds you all well,
I will be soob have to extract the nuclear protein Nrf2 from zebrafish embryos homogenate. I have to saperate the cytosolic protein from nuclear protein, which is the Nrf2. Can anyone refer me to such a protocol b/c most of the protocols I have found are either performed in cells or in rodents. I am looking for a protocol that is performed in zebrafish (Danio rerio) or an African clawed frog (Xenopus laevis). If someone knows of such a protocol, I would truly and sincerely appreciate the help.
Thank you very much, I truly appreciate.
Sincerely,
Omar
Hi,
I am including a reference to an article which I think might be useful. Kindly have a look.
Using hyperLOPIT to perform high-resolution mapping of the spatial proteome, Nature Protocols volume12, pages1110–1135 (2017)
Thank you.
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