Can I compare two mean scores of the same instrument, in the same sample? (Paired t-test)?

Hello everyone, I have scores on different scales of a sample of children, and I want to compare the mean scores on the different scales of this sample. For instance, my hypothesis is that...

10 December 2020 2,278 5 View

Protocol for proliferation of CD4+ murine T-cells?

23 July 2019 3,586 7 View

How to dissolve Dasatinib in order to go in vivo?

Dasatinib is an inhibitor of tyrosine kinases. I would like to test the effect of Dasatinib in vivo on my T cells. However, Dasatinib has a very low solubility (0.5mg/ml) with PBS, which is...

21 July 2019 3,056 3 View

Lysing of cell to detect antibody internalization?

07 April 2019 3,293 10 View

Fix 7-AAD staining?

Hi, I would like to make a viability staining using 7-AAD the day before the measurement; Is it possible to fix the cells? If yes, what do you suggest? Thank you!

01 April 2019 4,376 2 View

OT-I Rag1-/- genotyping protocol?

Dear all, Does anyone have a protocol to provide me to genotype OT-I Rag1-/- mouse? I have taken a look to OT-I and Rag1-/- genotyping protocols and I would need 4 primers for Tcra, 4 for Tcrb and...

10 December 2018 2,449 2 View

I need to measure the respiration rates in insects larvae. Do you have any suggestions about a feasible and not expensive equipment that can be used?

I know that there are avaialble some respiration chambers for insects but I was wondering if chambers used for small fruites can be adequate for the scope.

15 October 2017 3,205 2 View

What statistical analysis should I use for hierarchical questions data?

16 November 2015 9,532 4 View

Is it possible to change the orientation of hydrogen atoms of the ligand and protein residues? If yes, how is it possible by using Gromacs 5.0.4?

Dear users, I would like to change the orientation of an hydrogen of a D-serine (my ligand) and of a serine (as protein residue). How is it possible by using Gromacs 5.0.4? Thank you

12 September 2015 6,906 5 View

Could you please recommend me a manufacturer/ vendor of the GLAD(Glancing Angle Deposition) system?

Ideally we would like to buy a PVD equipment that can be used for the layer by layer and columnar growth.

09 June 2015 9,952 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

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01 March 2021 3,905 5 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

Which power-to-heat (P2H) devices do you think are likely to play the most crucial role in the European energy transition?

Power-to-heat (P2H) shows how we can use renewables for heating. Power-to-heat technologies have been on the market for quite some time now, with heat pumps being the most important. However, they...

10 February 2021 9,250 2 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View