We used to use Quantum333 media from PAA which was always great. Unfortunately they have been bought out by GE and GE, in their wisdom, no longer make this medium! We are currently trying Cascade Biologics Medium 106 from Gibco supplemented with FBS, glut and bFGF. While the cells look happy their doubling time is really slow compared to what we are used to.
Any recommendations welcome!
In our hands, DMEM with high glucose and 10% FBS has worked well to maintain cultures of human fibroblasts from various sources including the skin.
BASE: a-mem SERUM (FBS): 200ml/L L-GLUTAMINE: 10ml/L Pen/Str: 10ml/L
Works great with human fibroblasts.
Hi Michael, try the following, it has always worked for me when growing dermal fibroblasts:
Advanced DMEM (12491-015, ThermoFisher Scientific) + 10% FBS (F4135, SIGMA) + 1% Glutamax (35050-038 ThermoFisher Scientific) + 1% Anti-Anti (15240-062 ThermoFisher Scientific)
Hope this helps, Sam
DMEM (high glucose OK) with 10% FBS (HyClone, Characterized) and L-Glutamine or Glutamax works well. You can keep cells in 15% FBS for 1-2 passages after isolation.
Thank you all for your suggestions. We were kind of hoping someone would say that there is a direct equivalent to Quantum333, but I have passed on your recommendations to my colleague.
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