I'm working with in vitro phosphorylation and I would like to use a phosphatase inhibitor for my assay. Thank you very much!
Dear Alejandro,
You can use mutated forms of CPI-17 as a phosphoinhibitor for protein phosphatase 1.
http://www.ncbi.nlm.nih.gov/pubmed/12595264
Hoping this will be helpful,
Rafik
Ser/Thr, Tyr, and alkaline phosphatase inhibitors include sodium fluoride, sodium orthovanadate, beta-glycerophosphate, and sodium pyrophosphate.
Sigma has some phosphatase inhibitor cocktails, and this web page lists additional phosphatase inhibitors.
https://www.sigmaaldrich.com/technical-documents/articles/biowire/phosphatase-inhibitor.html
Information literacy in higher education integration with curricula engineering
04 August 2024 5,368 3 View
Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...
04 August 2024 8,432 3 View
I am working with electroluminescence (EL) imaging of photovoltaic panels and need to ensure that the modules are not damaged during testing. I understand that the bias current is usually set...
16 June 2024 5,514 1 View
Hi there, I have transfected these two plasmids into 293T packaging cells to encapsulate a pLVX-M-Puro coding plasmid. I had already validated the stability of the pLVX-M-Puro insert by transient...
06 June 2024 8,274 5 View
Please scientific articles on methodologies to create dashboards in R shiny and PowerBI
27 May 2024 387 1 View
Hello, these samples comes from Olinalá Formation (Permian age), Guerrero State, México. I need help to know if they correspond to Roadoceras, Stacheoceras and Waagonoceras (that have been...
27 May 2024 9,110 0 View
We are performing CRISPR on a gene from a strain of Aspergillus niger and then sequencing the results by Sanger. When we compare the gene sequence with the resulting sequence we observe in the...
25 April 2024 6,159 0 View
I am running simulations with oxdna using advanced techniques of sampling as Umbrella Sampling.
22 April 2024 5,897 3 View
Referring to the Article about challenges facing blood transfusion services in western Kenya, the question has come to mind as it is within the scope of my interest and in hopes that the answers...
10 April 2024 2,305 0 View
Hi there, I am planning an experiment to isolate the ER protein fraction from a cell culture. I have found a few commercially available kits, such as...
07 April 2024 2,979 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...
23 July 2024 8,492 3 View
Is there a method that I can measure alcohol dehydrogenase activity using NAD+ in enzyme extraction from plants?
17 July 2024 935 1 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
Hello, Does anybody have idea about any software or database that can give information about the possible phospho sites located in a protein? I am trying PhosIDP but its not working or loading...
15 July 2024 1,940 3 View
Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?
15 July 2024 2,055 3 View
any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...
13 July 2024 2,255 3 View
There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.
08 July 2024 4,988 1 View