I'm working with in vitro phosphorylation and I would like to use a phosphatase inhibitor for my assay. Thank you very much!
Dear Alejandro,
You can use mutated forms of CPI-17 as a phosphoinhibitor for protein phosphatase 1.
http://www.ncbi.nlm.nih.gov/pubmed/12595264
Hoping this will be helpful,
Rafik
Ser/Thr, Tyr, and alkaline phosphatase inhibitors include sodium fluoride, sodium orthovanadate, beta-glycerophosphate, and sodium pyrophosphate.
Sigma has some phosphatase inhibitor cocktails, and this web page lists additional phosphatase inhibitors.
https://www.sigmaaldrich.com/technical-documents/articles/biowire/phosphatase-inhibitor.html
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