Can anyone recommend a method for extracting phytochemicals from herbs and spices (mainly polyphenols and terpenes)?

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MH Zhou

Raymond Ming Wah CHAU PhD, The University of Hong Kong.

Chinese TCM herbalists are simply using 30-50% ethanol for a period of time, say 1 week to few months.

Jovale Vincent Tongco

You should have a copy of the book "Phytochemical Methods - A Guide to Modern Techniques of Plant Analysis" by J.B. Harborne if your research is focused on natural products chemistry and phytochemistry. Regards!

Serpil Kucuktepe

Cheers. I'm looking to concentrate the crude extract - would you suggest washing with a particular organic solvent to then rota vap? Or dry under reduced pressure?

Also, I have the book above however there does not seem to be one defined protocol. Do you have a page suggestion?

Stephen Karori

I have used HPLC for polyphenol assay. Check my publications.

Ashok Kumar Nadda

This article can be helpful for you

http://journal.frontiersin.org/article/10.3389/fpls.2016.01667/full

Best luck