Commercial amine-reactive fluorescent dyes are generally intended for derivatization of aliphatic amines. You should consult with an organic chemist on how to make the derivatives you want. In any case, can you make use of the natural fluorescence of the pterins?
Thank you for the answers.
It would be perfect for me if I could use the natural fluorescence of the pterins. Unfortynately, the equipment is the limitation. CE system works with LIF detector 480nm wavelenght of excitation.
The "amines in pteridines" are kind of special. Have you tried OPA? An alternative is an acid chloride attached to a fluorescent compound (acridine???)
Have you considered dansyl chloride? Not sure if it's applicable to your specific needs but it is a good fluorescent reagent for derivatising alkyl and aryl amines.
Thank you. I was thinking about OPA and dansyl chloride, but I abandoned this idea since I have found that their max of excitatnion wavelenghts are 334nm and 335nm, respectively. Our LIF dectector light source is 480nm.
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