Can anyone share a protocol for a ferrozine assay for tissue samples that is reproducible, including the lysis protocol? Also, what is the minimal amount of iron detectable using this protocol and which method was used to quantify the tissue amount (by protein?)?
Alexey A Tinkov
Dear colleague,
we estimate non-heme iron using the following method:
http://www.ncbi.nlm.nih.gov/pubmed/15026210
However, it describes the procedure for tissue homogenates, not cell lysates, and, hence, does not contain lysis protocol.
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