EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Can ethanol be used as an analgesic?

If ethanol has analgesic properties, why isn't it used for clinical purposes?

18 February 2021 8,227 4 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

How would I go about getting an expert review on the interview questions for the dissertation?

Good Evening, My name is David Marshall and I am Doctor of Management Student at Colorado Technical University Online. I just recently received approval from the IRB to start data collection on...

08 February 2021 346 7 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

How to plot Random Forest model as raster prediction map in RStudio?

Output_raster

27 January 2021 7,974 3 View

Requesting dataset for research purpose?

I am doing driving style analysis research with OBD data. Could anyone provide me links to get the dataset?I am not able to get it anywhere..

20 January 2021 9,707 2 View

Any suggestion of free of charge questionnaires that measure mood states?

I need a free of charge and short questionnaire that measures mood states. The questionnaire will be used as in a neuropsychological evaluation study. It is well known that mood states can...

16 January 2021 8,284 4 View

Too low protein concentration for Western Blot - any advice for polyacrylamide gel method?

Currently have punched mouse brain samples of different regions that are of too low protein concentration for Western Blot. Has anyone used the polyacrylamide gel electrophoresis method to detect...

09 January 2021 3,955 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

Which detection method is better for an ELISA-based assay: colorimetric or fluorometric?

Hello, I am planning to perform an ELISA-based assay to quantify the percentage of genomic methylation (5-methylcytosine) in human embryonic stem cells. I've been searching for some kits and I...

16 February 2021 8,389 3 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Hi, I have a problem with my mouse Adipose MSC primary culture?

I am working on mouse adipose mesanchymal stem cells. After removing the mouse peritoneal fat and extracting, I do the primary culture, I used DMEM F12 medium with 15% FBS, but with this, the...

13 February 2021 9,838 1 View

Which is the best way to calculate doubling time for human embryonic stem cells?

Hi! I would like to perform a doubling time assay in several newly derived human embryonic stem cell lines. Since hESC must be passaged as cell clumps, I cannot know the exact number of cells I am...

10 February 2021 2,895 4 View

What is the main difference between "CD105+ Endothelial Cells" and mesenchymal stem cells?

since CD105+ Endothelial Cells are CD31 negative, how do we differentiate them in cell culture from mesenchymal cells? is it using vwf? thanks a lot

03 February 2021 5,362 2 View

What is the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow endothelial cells?

Hello I am new to work on endothelial cells, and I have been reading about them a lot and trying to know the difference between human umbilical vein endothelial cells (HUVECs) and bone marrow...

28 January 2021 8,765 4 View