Mainly we find papers about an integrated approach to STEM/STEAM. I would like to better understand the role of the M beyond the mere use of its "tools".
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View
To generate curve but a straight line generated on zero load.
09 July 2024 2,617 1 View
Fellows, We are analysing data from force platform to calculate the power based on the acquired force. However, the current formula consider the distance covered by the individual as the "height"...
26 June 2024 2,049 1 View
How can I analyse the relationship between five independent variables and one dependent variable, with a moderating variable influencing each of these five relationships? How can I test hypotheses...
03 June 2024 6,221 1 View
I'm trying to stain areas of dystrophic calcification in sections of FFPE perinatal brain tissue. Typical basophilic appearance on H&E but nothing with Alizarin Red (costochondral junction...
28 May 2024 4,068 2 View
We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...
28 May 2024 3,529 1 View
We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....
28 May 2024 6,052 2 View
Hello. I am here looking for help with the NO radical scavenging assay. I was working on setting up the assay with sodium nitroprusside and the Griess reagent as they do in most works. However, I...
27 May 2024 5,013 1 View
Why we checking the GCA of a parent during hybridization program?
26 May 2024 590 1 View
at the time of filling the peroxide value is 0.5 however it increases with the passage of time. we have never faced such problem in past. please someone assist me in this regard.
22 May 2024 6,296 2 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...
06 August 2024 8,716 0 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hi. I have brightfield images of stem cells. I need to count the total number of cells/image. Some cells are individual and some cells are in colonies. Using average cross-sectional area of...
05 July 2024 4,197 1 View
Hi everyone, I've been working on differentiating human iPSCs to derive a pure and uniform culture of neural progenitor cells (NPCs). However, I'm encountering a significant issue during the...
01 July 2024 1,012 1 View
I need a PET + Solvent mixture to feed it to a fixed bed reactor for steam reforming.
30 June 2024 2,122 2 View
studi che esplorano l'interazione tra interculturalità, competenze digitali e educazione STEM/STEAM. Cerco esempi di progetti educativi o casi di studio che dimostrano come questi elementi possano...
24 June 2024 1,552 0 View
I know steam has become a potential for killing invasive plants. I am curious to know whether or not it has been used successfully in killing the plant and the seeds of Mile-a-minute (Persicaria...
19 June 2024 6,216 0 View