Can we use two antibodies generated in same species for indirect Sandwich ELISA?

I have generated two antibodies against two peptide fractions of a protein in the rabbit. I want to use these two antibodies (Ab-1 and Ab-2) in indirect sandwich ELISA for the detection of...

11 January 2021 1,754 4 View

How to coat plasma sample (Antigen) in ELISA plate for a direct ELISA?

I have generated an antibody against an antigen. Now, I want to use the developed antibody in the detection of the unknown sample. So, I want to coat the plasma sample as antigen. How that can be...

26 October 2020 2,259 7 View

Which path loss model will be suitable considering the scenario of factory automation with 5G URLLC?

In the performance analysis of modern wireless communication systems like the scenario of factory automation with 5G URLLC, which path loss model will be suitable and appropriate to assume? Any...

03 July 2020 918 2 View

How can i ensure the complete oxidation of graphite before quenching the oxidation process by H2O2?

I am following KMnO4 -H2SO4 reaction media for the synthesis of graphene oxide(GO) by an improved process.

04 June 2020 508 3 View

Does the SIMPLE algorithm solve the VOF single momentum equation as the descritized momentum equation, in free surface flow?

What is the link between VOF model and SIMPLE algorithm in CFD modeling ? How is the computing cycle works ?

10 February 2019 1,445 3 View

ALT and AST during hepatic damage - ELISA or Kinetic method ?

ALT and AST is released into the circulation during the hepatic damage. And hence, ALT and AST levels are reliable method to determine the hepatic damage. What is the drawback in using ELISA...

12 November 2018 5,636 1 View

How 5G Wireless Network can be Deployed with Existing 4G Infrastrucure?

As Time moves us towards the 5G Wireless Network...Needs Solution for Low Cost Technology for its Implementation.

02 May 2018 5,188 6 View

Hai ..any body please suggest OA journal with less fee and fast acceptance. I am totally confused with a great big list of open access.?

I need a good very less fee open acces (SCI or scopus) with good IF with less first review decision and too less rejection rate (sorry for giving more requisite) .I am from very poor back ground...

09 March 2018 9,141 5 View

How to pack fruit plants (EX .Avacado,Mango)to transport a long distance without having soil media.?What is the procedure..?

Here I am expecting the packing procedure to send to another country.

01 February 2017 1,100 3 View

What metabolic procedure can be considered for Type 1 Diabetes Mellitus?

17 August 2016 509 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View