I want to conduct an experiment for determining thyroid gland function. I came to know that both RIA and ELISA technique is used for this purpose among which ELISA is the economic one.
ELISA is the most comfortable technique than RIA. You can refer in "google" for protocol, type with catalogue number of the kit you are using. To get a general idea about procedure, you can always check "Cayman" and "Biocompare" kits. Yet, you should also consider about the reactivity and, intra and inter assay variations before proceeding. Good luck.
We used a commercially available RIA in our lab until it was discontinued a few years ago. We switched to an ELISA kit for T3 and T4 respectively, which have been working well so far. The protocols are attached. I hope that helps.
Our lab uses a T3 RIA from MP Biomedicals and a T4 EIA from Arbor Assays. Whichever kit you choose, make sure to validate properly. Conduct tests of parallelism and accuracy to ensure the kit is measuring what it should be measuring in your sample matrix. I've attached a PDF that describes ELISAs and the validations that should be performed.
http://www.arborassays.com/product/thyroxine-t4-enzyme-immunoassay-kit/
http://www.mpbio.com/product.php?pid=06B254215
When it comes to thyroid gland function (or "thyroid status" in a broader sense), plasma TSH levels and/or pituitary TSHbeta expression are sensitive parameters to indicatie hypo- or hyperthyroidism.
http://www.thyroidmanager.org/chapter/assay-of-thyroid-hormones-and-related-substances3/
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