I am seeking cheap sources of LH-20 and I would like to know if I can recycle it, and how many times is this feasible? How much would I need to buy it to complete a few structure elucidation studies et cetera. I have no experience with this as yet.
Sephadex LH-20 is useful for both analytical and industrial scales for the preparation of closely related molecular species. Due to the unique physico-chemical properties of
this medium, it can be used either during initial purification prior to polishing by high performance ion exchange or reversed phase chromatography, or as the final polishing step, for example, during the preparation of diastereomers.
Sephadex LH-20 is characterized by: • Unique chromatographic selectivity due to dual
hydrophilic and lipophilic nature of the matrix • Easily predicted elution behavior based on the chemical structure of the sample (1–3, 14) • Chemical and physical robustness,
• Though it is excellent and show batch-to-batch reproducibility. Sephadex LH-20 swells in water and a number of organic solvents. . Both the wet particle size
and exclusion limit for the medium vary depending on the solvent used for swelling, It can be recycled up to three-five times.
Yes, Sephadex LH 20 is expensive but you can use it many times, after you washed it with distilled water or methanol. And you can use many solvents for elution the components of your plant extracts , i.e. Methanol, Methanol: water in different relations, as well chloroform : methanol, chloroform : n-butanol: water (1:1:1) , etc,
May be you can ask to the Sephadex producer for some booklet to know little more about this gel filtration type.
I usually buy 100 g and recycle it, after washing with methanol I keep it in the column with methanol over it and don't let it dry out.
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