Protocol for Whole Blood Culture for Chromosome studies
This is the protocol we use in our lab for high resolution banding chromosomes. Good luck!
Dear Dr. Samartha
We have previously used the following protocol for metaphase analysis using whole blood:
"Peripheral blood was drawn from this donor and other healthy donors into heparinized vacutainers. Separate cultures were set up from each blood sample, using 1 ml blood in 9 ml RPMI 1640 medium (with 25 mM Hepes buffer and L-glutamine), containing 20% fetal bovine serum (Bio Whittaker) and 2.5% phytohemagglutinin (PHA, Gibco BRL). The cultures were incubated in dark, at 37 C in a humidified atmosphere of 95% air and 5% CO2."
I hope it helps.
The source is:
S.M.J. Mortazavi , T. Ikushima , H. Mozdarani . Variablity of chromosomal radioadaptive response in human lymphocytes.. International Journal of Radiation Research. 2003; 1 (1) :55-61
http://www.ijrr.com/browse.php?a_id=9&slc_lang=en&sid=1&ftxt=1
See paper of Ami et al.,, European Journal of Histochmistry, 2014, vol. 53, pp. 33-30
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