I am working on some ceramic samples with unexplained surface colour which I think may be linked to surface roughness. Can anybody explain?

We are working on some sintered ceramic samples that have different surface colours. Energy dispersive X-ray spectroscopy does not yield surface compositions that would explain a change in colour....

12 December 2019 3,778 3 View

Is this a unbalanced nested model? How should i go about analyzing this kind of design?

I am analyzing data from a field study. The main goal of this experiment is to understand what intervention can help increase students verbal reasoning. There are 4 kinds of interventions (say...

30 July 2019 7,037 5 View

What kind of factor analysis should i do on a questionnaire that has both ordinal and dichotomous items?

I am developing a scale that contains both ordinal and dichotomous items that are hypothesized to be affected by the same latent construct. For dichotomous items, tetrachoric correlation matrix...

08 May 2019 9,794 2 View

Can you reliably impute entirely missing surveys from a longitudinal study?

My colleagues and I have delivered a 4-wave survey (day 1, day 30, day 60, day 90), which makes use of multiple psychometric measures. We have the complete data for each survey collected, but also...

04 June 2018 8,786 5 View

Correlations and PCA for use in a SDM - use of background points?

Hello, I have a series of environmental data layers in r that I am trying to do a PCA on to reduce dimensionality for my SDM (species distribution model). I am attempting to do this in R in...

07 December 2017 8,591 7 View

When using RDA, why does my explanatory matrix explain a large amount of variation in my response matrix, but is not significant?

15 March 2017 5,983 3 View

How to culture the giardia?

How to culture the giardia. I know I need to use the TYI-S-33 medium. But it is too expensive in the atcc company. Where can I buy the diamond's vitamin solution? It's there any tips to culture...

25 October 2016 4,020 3 View

How to genotype E6.5 mouse embryos?

I am collecting embryos for histological analysis and need to genotype them beforehand. In the past I have worked with older mouse embryos (E8.5 and up), where it is easy to remove the yolk sac...

21 July 2016 8,584 3 View

How-to on running factorial manova?

21 April 2016 8,391 3 View

Has anyone ever done a protein filtration assay?

20 April 2016 8,349 8 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View