How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Is there any specific method or device to detect the metal in a deposit before excavation?

This will reduce the blasting and excavation cost during mining, as we will be excavating only those areas..

09 January 2020 4,581 11 View

I am a mining engineer and want to do M.Tech by research in Tunneling. Pls suggest me the interesting topics related to that?

My interest is rock mechanics and blasting.

05 June 2019 8,314 1 View

Can i claim my primer is specific for my microorganism?

I designed a pair primer when i blast in ncbi. blasting showed only in my microorganism it is enough for claiming specific a primer?

04 May 2019 3,722 6 View

What are some helpful resources to understand geophysics of soil for a mine blasting project?

I am conducting a project where underground blasting effects are analysed on soil and structures above the soil. I've currently only have a very basic Abaqus model of a soil block with an implicit...

04 May 2019 3,178 3 View

Dears, I am looking for Indian reviewer for a manuscript shall published in Geo-Marine Sciences (IJMS). if you are willing, please contact me?

Dear all kindly be advice that I'm looking for Indian reviewer to review the manuscript entitled ( Geological and mechanical assessment of nonmetallic minerals as blasting abrasive media for...

04 May 2019 3,721 1 View

Dears, I am looking for Indian reviewer for a manuscript shall published in Geo-Marine Sciences (IJMS). if you are willing, please contact me?

Dear all kindly be advice that I'm looking for Indian reviewer to review the manuscript entitled ( Geological and mechanical assessment of nonmetallic minerals as blasting abrasive media for...

02 May 2019 3,089 1 View

What are some helpful resources to understand geophysics of soil for a mine blasting project?

I am conducting a project where underground blasting effects are analysed on soil and structures above the soil. I've currently only have a very basic Abaqus model of a soil block with an implicit...

01 May 2019 7,958 2 View

How to design and verify specific primers for qPCR?

Hello all, I want to design specific primers to amplify a gene using qPCR. I already have many sequences geted by Blast method. I used Primer3Plus to design primers, then to verify specificity of...

12 March 2019 3,128 6 View

How to design and verify specific primers for qPCR?

Hello all, I want to design specific primers to amplify a gene using qPCR. I already have many sequences geted by Blast method. I used Primer3Plus to design primers, then to verify specificity of...

02 March 2019 9,833 10 View

When will a PCR lose efficiency or amplify unintended targets?

Hi, I've some questions I can't find answers for and hopefully someone over her can point me in the right direction. We have primersets against human targets, but our samples aren't clean...

01 February 2019 8,741 3 View