14 August 2021 1 996 Report

I'm trying to calculate the sample size I should have to study gene expression of IL-4 in asthmatic patients so I need a paper that is close to my work.

More Asmaa Mahmoud Abdelmaksoud's questions See All
Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View

What is the most accurate way to determine the energy gap of semiconductor materials? Is it better to use UV-Vis or PL measurements?

When preparing hierarchical zinc oxide ananostructure, the energy gap measured by PL was equal to (3.26 eV) While the energy gap value was determined by UV-Vis measurements (3.5 to 3.7 eV)

26 June 2024 7,505 6 View

Adding a frame element into an OpenSees model after running initial?

Hi, I am running an opensees model of a 2-bay 1-storey frame with settlement at the mid-support. I wanted to add an additional frame element as a bracing to the analyzed frame after it sustains...

08 June 2024 4,132 0 View

What is the most accurate artificial urine recipe used in vitro for microbiological purposes?

I just found many protocols but not specific for bacterial growth

30 May 2024 6,305 2 View

How can I explain the acid base titration test for a PH responsive nanogel?

I have three nanogels.. The difference between them is the cationic monomer ratio... However, the test is giving me similar curve of the three formulations

27 May 2024 2,064 0 View

Question about XRD?

Hello, I was elaborating thin films from FeCl3 using a dip coater. These films were deposited on a glass sheet for 6 layers and then annealed at 500°C. However, XRD analysis did not yield any...

31 March 2024 9,413 3 View

Can I use two IRIS in the same plasmid expression cassette ?

I am trying to express m6A protein and a fluorescence one RFP in the piggyback vector. I was thinking first of using the P2A to separate between the m6A and the RFP protein but then I was afraid...

12 March 2024 9,713 0 View

How can I encapsulate cisplatin into a ph responsive polymeric nanogel?

I have synthesised a 100 nm sized cationic nanogel and I want to use it as a carrier for cisplatin and paclitaxel

03 March 2024 3,426 0 View

Similar questions and discussions
Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

Cell culture model of Acute kidney injury?

Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...

29 July 2024 5,622 3 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

How to maintain intestinal tissue resident cells (TRM) in culture?

I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...

08 July 2024 4,887 2 View

Can i do protein expression of Toll-like receptor 4 cloned in pET-22B in E.coli cell?

Can

08 July 2024 6,725 4 View

IL-2 for PBMCs ex-vivo stimulation, best Concentration?

Hello everyone, I am isolating PBMCs and stimulating them using different stimulation conditions. my question is, I want to add IL-2 to the medium for better viability after stimulation, Would you...

16 June 2024 928 1 View

What is the potential role of phytochemicals in the prevention and management of asthma symptoms?

Asthma is a chronic respiratory condition marked by inflammation and narrowing of the airways. While traditional treatments involve medications like corticosteroids and bronchodilators, there's...

14 June 2024 8,063 2 View

Hi I want to make a DNA marcomolecule a flexible receptor in Autodock 4. However, I keep getting an error that prevents me so could someone explain?

I'm performing interactions between DNA as the macromolecule and Ethidium Bromide as the ligand. I'm looking to make the entire DNA molecule flexible in order to perform blind docking. However, I...

10 June 2024 6,270 1 View

Mouse MSCs not expressing TSG-6 on cytokine stimulation?

I am using mouse bone marrow derived MSCs (passgae 3-6) for my in vitro cytokine priming experiment. My basal media is 2% FBS-DMEM. I am using 10 ng/ml TNF-a, or IFN-g or both for 24 hours. I see...

27 May 2024 8,935 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close