Can anyone please help me with Protocol and Excel Template for Mitochondrial Copy Number Analysis via qPCR?

14 December 2024 0 9K Report

I am currently working on an experiment to analyze mitochondrial DNA copy numbers and perform bisulfite conversion using qPCR. I have a few queries and would greatly appreciate input from researchers experienced in this area:

qPCR vs. rtPCR for mRNA Expression: Is there a difference between qPCR for mitochondrial DNA analysis and rtPCR for mRNA expression? ( I need ct Valuse for analysis ). If so, what are the specific considerations for mitochondrial DNA quantification?

Protocol Request: Could anyone share a detailed protocol for performing qPCR specifically for mitochondrial DNA copy number analysis?

Excel Template for Ct Value Analysis: Does anyone have an Excel template for calculating and analyzing Ct values for qPCR experiments? Specifically, I need one that is tailored for mitochondrial DNA copy number calculations and bisulfite conversion analysis.

Your guidance and resources would be invaluable for my experiment. Thank you in advance!

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