I isolated a natural product from chloroform extract of my research plant, which is fluorescent in nature. Can any one tell me by analyzing the 1H and 13C spectra of compound that to which class of secondary metabolite this compound belongs?
Kindly help
Enclosure - NMR Spectra
with regard
Try to get molecular formula by performing High Resolution Mass Spec. It will help your analysis a lot.
LCMS would be good because this is obviously impure. In terms of NMR data you will need HSQC (ideally multiplicity edited) and HMBC data to assign this fully. At a guess, there looks to be a terminal alkene and an aldehyde.
Which plant? Do a literature search on that plant and related genus in case your compound was already found.
Agree with LC-MS, also check whether a porphyrin or chlorophyll was found.
Dear sir i am attaching the result of TLC analysis and HRMS, apart from UV-visible visualization i also used Iodine chamber, aniseldehyde reagent and methanol sulphuric reagent.
thanks to all for your kindness...
It's a nice job. I suppose another way that you can use CHNS elemental analyzer for analysing 1H and 13C in your project. This analyzer is capable of handling a wide variety of sample types, including
solids, liquids, volatile and viscous samples, in the fields of
pharmaceuticals, polymers, chemicals, environment, food
and energy.
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