Ajay Sharma

10 Questions 27 Answers 0 Followers

Questions related from Ajay Sharma

Can anyone kindly help me to characterized the unknown fluorescent compound isolated from chloroform extract?

I isolated a natural product from chloroform extract of my research plant, which is fluorescent in nature. Can any one tell me by analyzing the 1H and 13C spectra of compound that to which class...

08 August 2017 9,282 5 View

Is one can use its published text (in journals) in any chapter of his/her thesis?

i have publications in different journals. Can i used the text of my publication in my thesis.

07 July 2017 3,417 14 View

Can I re-use Diaion and Sephadex LH-20 for separation?

i want to separate polar plant extract on Diaion and Sephadex LH-20 and i want to know can i re-use Diaion and Sephadex LH-20? if yes then what is the procedure?

12 December 2015 9,513 4 View

How much of extract and resin (Diaion and Sephadex LH-20) should be taken for better separation?

i want to separate polar fraction of plant extract. For this i want to know how much amount of plant extract should be loaded in the column. Further, for batter separation what should be the...

12 December 2015 8,372 3 View

How to calculate the mg Fe (II) equivalent/g of dry weight in case of FRAP assay?

Standard equation of FeSO4 is y=4.5682x-0.0347. Different concentration used for the preparation of std curve are 60, 120, 180, 240, 300 mg/L. I took 100ul of sample having concentration 1mg/ml...

11 November 2014 929 6 View

What is the reason we estimate antioxidant activity of the same extract with different methods?

We estimate antioxidant activity of a extract with different method like DPPH, FRAP, Molybdenum reducing method and NO scavenging activity etc. which type of compounds responsible for these...

09 September 2014 4,039 9 View

What is the difference between iridoid and iridodial compounds and what are the different techniques used for their separation from plant extracts?

Which is the best method for their identification.

08 August 2014 5,650 4 View

How do I estimate phenolic and flavonoid content quantitatively in a plant extract?

I have used Folin–Ciocalteu reagent method for total phenolics (gallic acid as standard) and AlCl3 method for total flavonoid (catachin as standard) count estimation. In each method I have used 1...

07 July 2014 1,080 14 View

How to calculate total antioxidant capacity of Mo(VI) reduction assay?

I have used Mo(VI) reduction assay for total antioxidant capacity of plant extracts. i got the absorbance values, now how to calculate total antioxidant capacity of plant extract by using these...

07 July 2014 982 7 View

How long should Soxhlet extraction be run in order to have complete extraction of secondary metabolites from plant material?

I want to use Soxhlet method for the extraction of different extracts from plant material. but I don't know how long the Soxhlet extraction Should be run for complete extraction of secondary...

06 June 2014 4,710 16 View