Can anyone inform me about tools to be used in germ line mutation?

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Ahmed Housanie Elsappah

You can read these papers:-

1- Production of maternal-zygotic mutant zebrafish by germ-line replacement

https://www.pnas.org/content/99/23/14919

2- Talen Design and Screening Process to obtain fish with Germline Mutations in Gene of Interest.

http://www.mbl.edu/zebrafish/files/2013/03/Talen-assembly-and-screening-flow-chart.pdf

A. K. Shakur Ahammad

Thank you very much.