What are the methods or tools to be used in germ line mutation in fish?
You can read these papers:-
1- Production of maternal-zygotic mutant zebrafish by germ-line replacement
https://www.pnas.org/content/99/23/14919
2- Talen Design and Screening Process to obtain fish with Germline Mutations in Gene of Interest.
http://www.mbl.edu/zebrafish/files/2013/03/Talen-assembly-and-screening-flow-chart.pdf
Thank you very much.
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