Can anyone inform me about tools to be used in germ line mutation?

More A. K. Shakur Ahammad's questions See All

What are the tools to design random primer?

May I know the tools or software to design the random primer for PCR? Thanks in advance.

31 December 2018 7,240 4 View

How can I overcome of primer dimer?

Could you suggest what are the steps to overcome of primer dimer during PCR?

31 December 2018 6,735 12 View

Which enzyme is best for Real time PCR result?

Regarding real time PCR experiment, which enzyme (syber green or tag man) is convenient for getting best result? Thank you in advance.

31 December 2018 3,458 2 View

Could anyone provide carp fin tissue-genomic DNA banding pattern after gel electrophoresis?

What is the Genomic DNA banding pattern in carp fin tissue? Is it single band or smear? Could anyone provide this information? I will be grateful.

31 December 2018 115 1 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Ahmed Housanie Elsappah

You can read these papers:-

1- Production of maternal-zygotic mutant zebrafish by germ-line replacement

https://www.pnas.org/content/99/23/14919

2- Talen Design and Screening Process to obtain fish with Germline Mutations in Gene of Interest.

http://www.mbl.edu/zebrafish/files/2013/03/Talen-assembly-and-screening-flow-chart.pdf