A former member of my lab who has since moved on left behind a significant number of cell lysates for western blotting, already aliquoted into Laemmli's loading buffer ready for loading into gels.
Unfortunately, we do not know the concentration of the protein in the aliquots, so cannot use them as they are.
Therefore, does anyone know of a method for quantification of protein concentration in lysates that contain Lamemmli's? Something that's (obviously) not dependent upon colorimetric changes.