I want to determine colon-specific delivery ability of nanoparticles designed in our study in comparison with Eudragit-s nanoparticles loaded with budesonide. I tried 100ug BUD which is recommended dose (0.4mg/kg), collect GIT segments after 8 hours and extract with methanol and analyse but not detected by HPLC, most probably BUD is degraded as reported in different studies. Can I use a high dose to make easy for analysis? Your suggestion will be appreciated.
I think you could experiment with up to two times the recommended dose. Maybe even more since it is a steroid, but not too much more. I would increase the administered dose by 0.1 mg/kg in successive trials if you are trying to find a good dose that does not produce bad side effects and perform the analysis at the same time. This is just a guess, but it is my two cents.
Thanks Cory, I tried some high doses but not fruitful, only the stomach samples gave peaks while other GIT segments didnt, i dont know where the drug goes, may be degraded inside live rat GIT or during extrarction process, I am still trying to optimise the conditions
Maybe you could try rectal delivery if you are trying to get budesonide in the colon. This might defeat the purpose of the study, but it would probably work.
Thanks again. We are working on orally administered colon specific nanoparticles drug delivery, the alternative of BUD is C-6 to determine the ability of nanoparticles for colon-specific delivery.
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