I need to extract RNA from soil samples, however my samples are stored at -20 ° C and without soil preservation solution for about 3 months. Will it be more prudent to resample soils or it is okay to use this frozen sample? The objective is to quantify RNA per gram of soil by spectrophotometric methods.
Birgit Pfeiffer
Hi Carolina,
to my experience storage of samples aimed for RNA extraction at -20°C is not advisable.
We store our samples at -80°C without any preservation solutions and get very good RNA extracts after month, while RNA extracts from samples stored at -20°C showed only low concentrations or even no RNA content. Due to the expectable high concentration of RNases present in soils, as well as their resilience.
With regards Birgit
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