Carolina Parelho

4 Questions 7 Answers 0 Followers

Questions related from Carolina Parelho

Which filter should I use to perform the AO in sperm cells?

I am performing the AO in frozen sperm cells from wild mice. I have stained and visualized in a Zeiss fluorescence microscope with set 38 (ex 470/40, beam splitter 495 and emiss 525/50). I only...

12 December 2014 3,528 7 View

How do you determine the age of wild mice?

Does anyone here work with wild mice (mus musculus)? I would like to know how to determine the age of these animals? Lens eye weight? Currently I am using the fresh body weight, but this...

06 June 2014 8,413 4 View

Is it okay to sieve soil samples before freezing in liquid nitrogen prior to RNA/DNA extraction?

I will co-extract RNA and DNA from soil (andosols). Taking into account the difficulty of extracting good yields of nucleic acids in these particular soils, is it okay to sieve them before...

02 February 2014 7,254 5 View

Can anyone help with RNA isolation from soil samples and samples storage?

I need to extract RNA from soil samples, however my samples are stored at -20 ° C and without soil preservation solution for about 3 months. Will it be more prudent to resample soils or it is okay...

02 February 2014 2,224 2 View