Can anyone help with rescuing antibody CDR sequences from hybridoma supernatant?

20 February 2014 1 3K Report

Is it possible to clone the CDR sequences of a monoclone from hybridoma supernatant? Unfortunately my promising clone died and I have only a limited amount of hybridoma supernatant left.

Alexandre Fontayne

The only strategy I see is purify your mAb then perform MS analysis on the mAb Under reduicing condition to analyse separately the heavy and light chain and get the amino acid sequences out of them. Then by using databases such as IMGT, I guess you should find what you are looking for.

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