I'm trying to express PCV2 VP2 protein in E.coli with high biologic activity. I'v tried to express it with many fusion proteins in different host strains under levels of temperature , however I always get inclusion body.
I want to get 5-10 micrograms of linearized plasmid for Pchia pastoris' electrophoretic transfer, 20 micrograms of plasmid is linearized and then dephosphorylated, 2.5 volume of ethanol is...
11 December 2015 5,548 4 View
I have got some dead chickens suspected to virus Ankara, however, I failed to find related papers on detecting virus Ankara, either design good primers. Could anyone do me a favor to give the...
11 December 2015 4,620 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
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05 August 2024 4,407 0 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
Hello everyone, I am try to understand the differences interaction between insoluble and soluble polymers in water with metal ions and how theit charge density (of metal ions) affect the interaction.
29 July 2024 6,350 3 View
Dear all: I am modifying Bismuth Tungstate with Hematite (Fe2O3), so I would like to know about how much Iron(+3) can form a solid solution in a Bismuth Oxide matrix. Best wishes Marco
29 July 2024 2,163 1 View
I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...
28 July 2024 2,979 1 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View