Lide Qin

3 Questions 1 Answers 0 Followers

Questions related from Lide Qin

Can anyone help to recommend any software for analysising the solubility and aggregation trend of a protein or a region when expressed in E.coil ?

I'm trying to express PCV2 VP2 protein in E.coli with high biologic activity. I'v tried to express it with many fusion proteins in different host strains under levels of temperature , however I...

10 October 2017 5,726 0 View

How can I gather linearized plasmid effectively?

I want to get 5-10 micrograms of linearized plasmid for Pchia pastoris' electrophoretic transfer, 20 micrograms of plasmid is linearized and then dephosphorylated, 2.5 volume of ethanol is...

12 December 2015 5,521 4 View

Can anyone provide me a pair of PCR primers for detection of virus Ankara?

I have got some dead chickens suspected to virus Ankara, however, I failed to find related papers on detecting virus Ankara, either design good primers. Could anyone do me a favor to give the...

12 December 2015 4,589 2 View