I have to extract the RNA and the protein, but not the DNA,

I have main problem with solibilising the protein pellet at the end, anyone have better ideas what to do? Thanks

Heres my protocol :

RNA Extraction and Protein Extraction 

Add 700ul Trizol to the sample, mix vigorously

Incubate the homogenate at room temperature for 5 mins

Add 140 ul chloroform and cap tube securely. Shake vigorously for 15s

Incubate at RT for 2 mins

Centrifuge for 15mins at 12000g at 4°

Transfer the upper aqueous phase (~400ul ) to new tube. (BL) Avoid transferring any interphase.

The remaining phase (~450ul) used for protein extraction. (BL)

RNA extraction-

Add 1.5 volumes (~600ul) of 100% commercial ethanol, and mix thoroughly by pipetting.

Pipet up to 700ul sample, including precipitate, into an RNeasy MinElute spin column in a 2 ml collection tube. (BL)Close the lid and centrifuge at >8000g for 15 s at RT. Discard flow-through.(FT)

Repeat step 8 using the remainder of the sample

Add 700ul buffer RWT to spin column. Close lid, centrifuge at >8000g for 15s at RT. Discard FT

Add 500ul Buffer RPE onto spin column. Close lid, centrifuge at >8000g for 15 s. Discard FT

Add 500ul 80% ethanol to spin column. Close lid, centrifuge at >8000g for 2 mins. Discard FT

Place the spin column in a new 2ml collection tube (supplied).

Open the lid centrifuge at 14000g for 5mins to dry the membrane. Discard FT +collection tube

Place the spin column in a new 1.5 collection tube (L)(supplied).

Add 14ul RNase free water directly in the center of the spin column membrane. Close lid, centrifuge for 1 min at 14000g to elute the RNA

Protein extraction-

A: Precipitate the DNA

Remove any remaining aqueous phase overlying the interphase

Add 210ul of 100% ethanol

Cap the tube, mix by inverting the tube several times

Incubate for 2 mins

Centrifuge for 5 min at 2000g at 4° to pellet the DNA

Transfer the supernatant to a new 2ml Eppendorf tube for protein isolation (or store at -70 for future use)

B: Extract the Protein

Add 1050ul of isopropanol

Incubate for 10mins

Centrifuge for 10mins at 12000g at 4°, discard supernatant

Prepare a wash solution with 0.3 M guanidine hydrochloride in 95% ethanol (eg.0.084g GHCL per 3000ul ethanol)

Resuspend the pellet in 1.4 ml (1400ul) of wash solution

Incubate for 20 mins

Centrifuge for 5 mins at 7500g at 4°

Discard the supernatant with a micropipettor

Repeat resuspension and centrifugation twice.

Add 2 ml of 100% ethanol, then mix by vortexing briefly

Incubate for 20 mins

Centrifuge for 5 mins at 7500g at 4°

Discard the supernatant with a micropipettor

Air dry the protein pellet for 5-10 mins

Incubate the sample at 50°heat block

Resuspend the pellet in 200ul of Bio-Plex cell lysis buffer by pipetting up and down

Centrifuge for 10mins at 10000g at 4° to remove insoluble materials

Transfer the supernatant to a new tube, store at -20°