I am trying to do some measurements with millipore scepter sensors. However too often while measuring the apparatus stops by the end of the run with the message "low start". And I can´t guess what the problem is...
Valeria,
We had many problems (sensor block or low start) with Millipore Scepter. I think, the product failed. If you have two many samples, flow cytometer might be an option. If you have few samples, manual staining kit might be an option.
I have just found a way to overcome my problems and i want to share it:
I started to turn the pipette upside down during measurement and in this way i stopped losing sensors. there is a video in this review of the scepter that explains how to do it
http://ucflow.blogspot.com.ar/2011/10/counting-cells-with-emd-millipore.html
i hope it will be usefull for others to!
I have been trying to use scepter for counting spores in the size range of 2 to 6 um. (belloniging to a microsporidian in the gut of bees). Almost all my sensors exhibit a "lost start". As I can not see any bubbles in the sampling area of the sensor, I have been thinking this can be a consequnese of blocking. So far, at considerable dillutions and centrifugation at 500 rpm (removal of tissue components) I can confirm the spores remain in the supernatant via microscope, but the "lost start" issue persists. Also, turning upside down does not solve the problem (I can measure the reference beads with no problems). Does somebody know, if there is some issue with various components (e.g. protein, lipids ...) that this sensor has a problem with?
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