Can anyone help me with column chemistry and provide details for HPLC analysis of drug substances and product?

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Wangkheirakpam Sujata

START WITH C18. SEE ACCORDING TO POLARITY CLASSIFICATION OR IF U WANT TO SEPARATE ENENTIOMERS GO FOR CHIRAL COLUMN

Rahul Dahale

ok thank you sujataji, but i have problem that my compound elute late on C8 column and earlier on C18 in Water ACN mixture have around 12-13% carbon load on column whats the problem although compound is lipophillic

you decrease flow rate in C18 and see