I need help regarding how a compound undergoing separation in a column is affected by the column properties.
START WITH C18. SEE ACCORDING TO POLARITY CLASSIFICATION OR IF U WANT TO SEPARATE ENENTIOMERS GO FOR CHIRAL COLUMN
ok thank you sujataji, but i have problem that my compound elute late on C8 column and earlier on C18 in Water ACN mixture have around 12-13% carbon load on column whats the problem although compound is lipophillic
you decrease flow rate in C18 and see
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